
In vitro cultivation of stone pine trees with highly cone-producing clones of pine nuts
The objective of this project is to develop an in vitro stone pine growing system based on clones that are highly productive of pine cones and to see if the growing trees in the nursery keep the productive potential of female flowers and pine nuts from a young age.

development of a rapid DNA test for determination of Tuber species
At Micofora we analyze more than 300 kilos of Truffles annually, one by one. The aim is to develop a rapid system for molecular determination of different varieties of Truffles, which will later be used as inoculum in the substrates for Truf-Up truffle nests and in the nursery.

Development of the cultivation of the white italian truffle Tuber magnatum
The objective of this project is to develop the technologies and protocols for the production of truffle trees with the white truffle Tuber magnatum. For this, the technologies have been adapted in our molecular laboratory for the detection of mycorrhizae and mycelium in the soil of this truffle. As a result of this work, Micofora [...]

technology transfer for the production of truffle trees in New Mexico (US)
Technology and know-how transfer and training project to build greenhouses to produce large scale Truffle trees for the American market. Nursery created in Las Cruces, New Mexico. Rovirosafarms.com nursery

research on drip irrigation in truffle farming to optimise water consumption
research on drip irrigation in truffle farming to optimise water consumption and how to adapt to climate change. The production results in several campaigns show how improvements are achieved in the size of the truffle fruitbodies with a saving of 2/3 of water consumption compared to traditional micro-sprinklers. We used moiesture, temperature, conductivity and VPD [...]

R&D project on the use of mulching to reduce evapotranspiration in truffles
3 treatments are tested: white fabric geotextile, rice husk and pruning branches to compare the temperature, EVTP, moisture and conductivity in the soil and how the different treatments affect the production and size of harvested truffle fruit bodies.
Steam weed control trials in trufficulture
A steam machine is tested for ecological control of weeds in trufficulture. Temperature measurements are taken at different depths to evaluate its effect on the truffle mycelium. The conclusion is that at the standard speed and the temperatures reached in soil a few centimeters down, do not affect the development of the Tuber mycelium.

Production and study of mycorrhizal pecan trees for truffle and nut production
With the aim of producing mycorrhizal pecan trees for our nurseries in NM, USA. Trials are being developed for large-scale commercial production of mycorrhizal pecan (Carya illinoinensis) for truffle production. We tested and obtained mycorrhizal plants with Tuber melanosporum, borchii and aestivum and monitored mycorrhization in the field.

Mature hazel nut inoculations with truffles
Start date: octubre 2006 Objectives: Evaluate the potential to inoculate on mature hazels nut orchards with truffles (Tuber melanosporum & Tuber brumale). Location Castellon and Tarragona. Description: Inoculations were done in more than 4000 hazels with Tuber melanosporum & Tuber brumale. Subsequently it was followed by mycorrhiza analysis. Finally also we evaluated the production [...]

R+D Project To Develop A Truffle Farming Fertilizer
Start date: March 2016 Objectives: To test the truffle mycelial quantification using different treatments with organic products and microorganisms in nursery and truffle plantations. Methodology: For two years will establish several trials using different organic and microorganisms products were applied in nursery and truffle plantations. Subsequently we´ll monitoring the mycelium concentration by qPCR to assess [...]

Pine Mycorrhized With Lactarius – Mushroom Fruiting on Pots
Start date: March 2016 Objectives: This project aims to achieve the systematic production of Pinus pinea mycorrhizated with a special strain of Lactarius deliciosus, able to bear fruit in the pot from the second year of age. Description: For 3 years will make 3 production cycles with stone pine infected with saffron milk cap [...]

Mycoforest Technology – Nueva Zelanda
Start date: June 2006 Objectives: Technology tranfer to inoculate radiata pine (Pinus radiata) forests with Lactarius deliciosus and Boletus edulis edible wild mushrooms. Design and construction of digger-injector for direct inoculation into forest. Description: Mycoforest technology to be used on a large scale was adapted. For 4 years saffron milk cap and porcini mushrroms [...]
Growing truffles on Pines – Mexico
Start date: September 2013 Objectives: To evaluate the ability of a farm in Mexico to cultivate truffles, transfer technology to produce mycorrhizal plant, assess the feasibility of a range of local pine (Pinus cembroides) for cultivation of Tuber bochii and make a pilot plantation. Description: In a first phase we analyzed the potential of [...]

Technology Transfer for Mosbec Truffle Farms – South Africa
Start date: March 2013 Objectives: Technology Tranfer for production of mycorrhizal tree nursery with black truffle (Tuber melanosporum) in South Africa. Description: In a first step a potential for growing truffles in South Africa map was drawn, suing Geographical Information Systems (GIS). Then it began to transfer the technology to produce trees mycorrhized with [...]

Research Project on Bacteria Associated to Truffles
Start date: January 2009 End date: December 2011 Microbiological analysis in rhizosphere of seedlings and truffle trees for possible commercial application: a grant Torres Quevedo PTQ-08-03-07858 was obtained. Description: Using a special medium designed by Dr. Xavier Vilanova, Pseudomonadaceae populations were isolated from wild truffieres over 1 year. Additionally they were characterized by PCR and [...]

Rural Development by Growing Mushrooms in Nicaragua.
Date: june 2006-2008 In Rivas, Nicaragua. To identify mushroom production initiatives and training communities of women to establish production farms to grow mushrooms. Technology transfer to produce spawn; to develop a substrate based on farming waste products (from sugar cane, rice...); facilities construction and farming management. More information More information [...]